It is our great pleasure to introduce a Proteomics Webinars Series for “A Tour of Switzerland”. Organized by the LS2 Proteomics Section, these virtual sessions will bring you to different cities in Switzerland to discover what Proteomics is all about there.
Basel's Webinar - March 4th - 10h30/12h00
“Isobaric tagging – a versatile quantitative proteomics approach for academic and industry needs”
In this webinar we want to provide insight of the very different proteomics research activities taking place in Basel. As one of the biggest life-science hubs in Switzerland, a large number of mass spectrometry-based proteomics labs have been established in and near Basel to support clinical and basic research activities in both academia and industry. With proteomics becoming an indispensable screening tool for targets of interest within complex human samples, there is a strong need for high-throughput and global quantitative proteome analyses. Isobaric tagging technologies fulfills these needs to a large extend and has proven useful for many challenging applications. With the help of three selected projects, we will give an overview of the versatility of isobaric tagging regarding biological and pharma applications, but also discuss remaining challenges, in particular for large-scale screens.
1/ Introduction to TMT (10 minutes)
- Loïc Dayon, Nestlé Research: A brief history of isobaric tagging with tandem mass tags
2/ Applications (15 minutes each, questions at the end)
- Jan Seebacher, FMI: Determining the bite-size and substrate specificity of the Dipeptidyl peptidase IV family protease DPF-3 using TMTpro
- Katarzyna Buczak, Biozentrum, University of Basel: Breaking the 16-sample barrier - high throughput screening with multi-batch TMT
- David Avila, Hoffman La Roche: Automated and multiplexed Oligo-Competitive Capture Mass Spectrometry (OCC-MS)
Organizing Committee: Emmanuelle Lezan, Manuel Tzouros, Alexander Schmidt
Moderator: Alexander Schmidt - Biozentrum, University of Basel
Visit the LS2 Proteomics section webpage here https://www.ls2.ch/sections/proteomics
