The 14th Microscopy Webinar will take place on December 13th from 16:00 to 17:00 (Swiss time)
Speaker: Roman Renger - Lumicks (The Netherlands)
Title:Towards Single Molecule Biology using Optical Tweezers and Fluorescence Microscopy
Abstract: Biological processes occur across various time and length scales, connecting molecular details with collective, emerging phenomena. Detailed insights into the most fundamental building blocks of life such as individual proteins and nucleic acids provide essential information for understanding the fundamentals of intra- and extracellular organization. Direct, real-time observations of biomolecular interactions are required to validate and complete the current biological and biophysical models.
Single-molecule technologies offer an exciting opportunity to meet these challenges and enable researchers to study molecular function and activity in real-time. Here, we present our efforts towards further enabling discoveries in the field of biomolecular interactions by correlating optical tweezers and confocal fluorescence microscopy. We present several examples in which our technologies enhanced the understanding of membrane-less compartments, genome organization and biomolecular folding. Furthermore, we show how advances in hybrid single-molecule methods can be turned into an easy-to-use and stable instrument that has the ability to open up new avenues for breakthrough discoveries in biology and biophysics.
Biological processes occur across various time and length scales, connecting molecular details with collective, emerging phenomena. Detailed insights into the most fundamental building blocks of life such as individual proteins and nucleic acids provide essential information for understanding the fundamentals of intra- and extracellular organization. Direct, real-time observations of biomolecular interactions are required to validate and complete the current biological and biophysical models.
Single-molecule technologies offer an exciting opportunity to meet these challenges and enable researchers to study molecular function and activity in real-time. Here, we present our efforts towards further enabling discoveries in the field of biomolecular interactions by correlating optical tweezers and confocal fluorescence microscopy. We present several examples in which our technologies enhanced the understanding of membrane-less compartments, genome organization and biomolecular folding. Furthermore, we show how advances in hybrid single-molecule methods can be turned into an easy-to-use and stable instrument that has the ability to open up new avenues for breakthrough discoveries in biology and biophysics.
