The 9th Microscopy Webinar will take place on May 31st from 16:00 to 17:00 (Swiss time)
Speaker:
Orane Guillaume-Gentil – (Institute of Microbiology, ETH Zurich, Switzerland)
Title: Live single-cell analysis by fluidic force microscopy
Abstract: While single-cell analysis is revolutionizing biological sciences, the development of technologies enabling the non-destructive manipulation and examination of single cells is in demand. Fluidic force microscopy (FluidFM) combines nanofluidics, atomic force microscopy and optical microscopy into a miniaturized pipette matching the scale of individual cells.1 Operating with subcellular precision and capable of handling pico to femtoliter volumes, FluidFM allows for the selective perturbation and analysis of individual live cells within physiological environments. Over the years, we developed the technology for a variety of compementary applications, ranging from spatial manipulation and mechanical measurements, to the cellular injection and extraction of biomolecules and organelles.2,3,4 In recent studies, we coupled the sampling of endogenous molecules by FluidFM to high sensitivity molecular analyses.5,6 Keeping the cell in its spatial environment, alive and functional for later assessment, the approach provides the means for temporal and spatially-defined single-cell analysis, advancing the frontiers of single-cell omics.
- Meister, Gabi et al. Nano Letters 2009, 9:2501
- Guillaume-Gentil et al., Trends Biotech 2014, 32:381
- Guillaume-Gentil et al. Cell 2016, 166: 506
- Gäbelein et al. PLoS Biol 2022, 20(3): e3001576
- Guillaume-Gentil, Rey et al. Anal Chem 2017, 89:5017
- Chen, Guillaume-Gentil et al. Nature 2022, in press (bioRxiv)
